This study presents a modified method for analyzing the InDel (I/D) polymorphism of the ACE1 gene using temperature melting curve analysis of PCR products. The ACE1 gene plays a critical role in regulating blood pressure and electrolyte balance. The InDel polymorphism of ACE1 is linked to various pathological conditions, including COVID-19. The method developed in this research enables efficient genotyping by examining melting temperature curves, offering a reliable and simple approach for detecting genotypes. The method’s effectiveness was validated using specific primers and real-time PCR equipment, allowing the identification of homozygous deletion, homozygous insertion, and heterozygous genotypes.
Takeaways:
- The modified genotyping method offers a simple and reliable approach for detecting InDel polymorphism in the ACE1 gene.
- The method uses temperature melting curve analysis, which is an efficient way to differentiate genotypes based on the melting temperature of PCR products.
- The method can help in the analysis of genetic markers associated with cardiovascular diseases and the prognosis of COVID-19.
- This technique is cost-effective, requiring only one pair of primers for accurate genotyping.
- The use of this method could improve clinical practices by offering a faster, reliable tool for genetic diagnostics.
